Analysis of the protein profile of the venoms of snakes Bothrops asper, Bothrocophias myersi and Crotalus durissus from the Colombian Andean Region obtained by RP-HPLC

Snake venoms comprise a highly complex mixture of proteins, and there is also high interspecific intraspecific variability in their composition, even the same region. Our aim was to compare composition Bothrocophias myersi, Crotalus durissus Bothrops asper, snakes from Andean region Colombia by Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC). The were given research group under an agreement with Fundación Zoológica de Cali. pool obtained manual extraction, lyophilized refrigerated. protein found quantified spectrophotometry using Bradford Lowry methods direct measurement Nanodrop®. stablished RP-HPLC, Lichosper 100 RP, C18 column (250X4 mm) pore size 5µm, as well Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). highest quantity venom B. myersi (108,6 mg/mL) followed C. (78,1 asper (74.1 mg/mL). All showed bands 15 50 KDa SDS-PAGE; most important finding abundance PLA2 svMP myersi. Chromatographic analyses revealed very similar profile, but certain differences toxins abundance. We conclude that process separating proteins RP-HPLC SDS-PAGE are first step know profiles, which turn could allow medical staff elucidate clinical syndrome produced snakebites.